«Scientific Journal of the Far Eastern State Technical Fisheries University». Vol. 45. No 2. 2018 – download pdf


ICHTHYOLOGY. ECOLOGY


V.N. Kazachenko
Far Eastern State Technical Fisheries University
Russia, Vladivostok

I.V. Matrosova
Far Eastern State Technical Fisheries University
Russia, Vladivostok

Ya.A. Shakhova
Far Eastern State Technical Fisheries University
Russia, Vladivostok 

REPRESENTATIVES OF PARASITIC CRUSTACEANS (CRUSTACEA) OF LUNE-FISH MOLIDAE (TETRAODONTIFORMES)

Abstract. The data on parasitic crustaceans of the Molidae family are presented: Copepoda – Trebius sp.,Pandarus bicolor, Dinemoura producta, D. latifolia, Echthrogaleus coleoptratus, Cecrops latreilli, Orthagoriscicola muricatus, Philorthagoriscus serratus, Caligus elongatus, C. rapax, Lepeophtheirushastatus, L. nordmanni, Anthosoma crassum, Pennella filosa, Lernaeopoda bidiscalis; Argulidae – Argulisscutiformis; Lepadidae – Conchoderma virgatum; Isopoda – Nerocila orbignyi.

Keywords: parasitic crustaceans, lune-fish.


Halyna Tkachenko
Department of Zoology and Animal Physiology, Institute of Biology and Environmental Protection, Pomeranian University
Slupsk, Poland

Lyudmyla Buyun
M.M. Gryshko National Botanical Garden, National Academy of Science of Ukraine
Kyiv, Ukraine

Olha Kasiyan
Danylo Halytsky Lviv National Medical University
Lviv, Ukraine

Elżbieta Terech-Majewska
Department of Epizootiology, University of Warmia and Mazury
Olsztyn, Poland

Zbigniew Osadowski
Department of Zoology and Animal Physiology, Institute of Biology and Environmental Protection, Pomeranian University
Slupsk, Poland

THE ANTIBACTERIAL ACTIVITY OF THE ETHANOLIC LEAF EXTRACT OF FICUS PUMILA L. (MORACEAE) AGAINST FISH BACTERIAL PATHOGENS

Abstract. The aim of this study was to test the antibacterial properties of ethanolic extract prepared from Ficus pumila L. leaves against fish pathogens, Aeromonas hydrophila, Citrobacter freundii, Pseudomonas fluorescens,Yersinia ruckeri to evaluate the possible use of this plant in preventing infections caused by thesebacteria in aquaculture. The antimicrobial susceptibility testing was done on Muller-Hinton agar by discdiffusion method (Kirby-Bauer disk diffusion susceptibility test protocol). Muller-Hinton agar plates wereinoculated with 200 and 400 μL of standardized inoculum (108 CFU/mL) of bacterium and spread withsterile swabs. Aeromonas hydrophila (strain E 2/7/15) isolated locally from gills of rainbow trout (Oncorhynchusmykiss Walbaum) and Pseudomonas fluorescens (strain E 1/7/15) isolated locally from internalorgans of rainbow trout with clinical features of furunculosis, Citrobacter freundii isolated locallyfrom gills of eel (Anguilla anguilla L.) with clinical features of disease, as well as Yersinia ruckeri collectedfrom clinically healthy fish and fish with clinical symptoms of yersiniosis were used as test organisms.In our study, the A. hydrophila and C. freundii strain (200 and 400 μl of standardized inoculum)revealed intermediate susceptibility to ethanolic extract obtained from leaves of F. pumila (inhibition zonediameters ranged from 10 to 11 mm). Ethanolic extract derived from F. pumila leaves exhibited the highestantibacterial activity against Pseudomonas fluorescens causing a zone of inhibition, comprising atleast 13-14 mm for 200 μL and 9-10 mm for 400 μL of standardized inoculum (108 CFU/mL) of bacteriumstrain. Y. ruckeri isolate (200 and 400 μl of standardized inoculum) revealed high susceptibility to ethanolicextract obtained from leaves of F. pumila (inhibition zone diameters ranged between 12 and 14 mm).Further studies aimed at the isolation and identification of active substances from the ethanolic extractsobtained from leaves of F. pumila could also disclose compounds with better therapeutic value. It is believedthat screening of plants from Ficus genus for other biological activities including antimicrobialactivity is essential. Finally, it seems important not only to determine the substances involved in the activityagainst pathogens but also to establish the induced response in the fish physiology.

Keywords: Aeromonas hydrophila, Citrobacter freundii, Pseudomonas fluorescens, Yersinia ruckeri, antimicrobial activity, disc diffusion technique, ethanolic extract.


Halyna Tkachenko
Department of Zoology and Animal Physiology, Institute of Biology and Environmental Protection, Pomeranian University
Slupsk, Poland

Joanna Grudniewska
Department of Salmonid Research, Stanislaw Sakowicz Inland Fisheries Institute 
Żukowo, Poland

TISSUE-DEPENDENT CHANGES OF AMINOTRANSFERASES ACTIVITY IN GRAYLING (THYMALLUS THYMALLUS LINCK) AFTER CHLORAMINE-TDISINFECTION

Abstract. In this study, we have tested use of Chloramine-T in dose 9 mg per L in disinfective procedures in the grayling (Thymallus thymallus Linck). To observe the effects of Chloramine-T at the therapeutic concentration,grayling, which is one of the most important salmonids in human diet was studied. Further, theaim was to evaluate the safety of this disinfective product (recently has been attracting researchers’ attentionfor use in aquatic animals) for fish health using markers of aerobic and anaerobic capacity in thegills, muscle, hepatic, and cardiac tissues of grayling. The alanine (ALT) and aspartate aminotransferases(AST) activity were assessed in various tissues of grayling exposed to Chloramine-T and compared to untreatedcontrol. In the present study, ALT activity after exposition of Chloramine-T was significantly decreasedby 10% (p=0.005) in the cardiac tissue of the fishes when compared to control value. Muscle andgill tissues also showed similar results as in cardiac tissue such as decrease of ALT activity by 18% and6.4% (p>0.05), respectively. In case of hepatic tissue, ALT activity was non-significantly increased comparedto control. The results clearly depicted that ALT activity was raised lowly in the cardiac tissue ofgrayling but this decreased activity was not tissue specific. AST activity was increased significantly in thehepatic tissue by 19% (p=0.007) but decreased in the cardiac tissue by 16.7% (p=0.002) as compared tocontrol value during Chloramine-T disinfection. AST activity in the muscle tissue and gills afterChloramine-T exposition showed usual trend of decreased as compared to control value. In the presentstudy, liver tissue showed the highest elevation of AST activity, while, in heart, the decrease of AST activitywas observed. In case of muscle and gill tissues, non-statistically decreased AST activity was 20% and7%, respectively. Considering all the tissues, here, the increase of AST activity was observed in the liver(by 19%, p=0.007) and the decrease in the gills, muscle and cardiac tissues under Chloramine-T exposure.The study also showed that hepatic and cardiac tissues were more sensitive to the changes than gillsand muscle tissue. Therefore, these biochemical parameters can be considered as indicators for assessmentof disinfective effects, although further studies are required for investigating the mechanism involvedin this pattern. This study opens a new perspective on the investigation of toxic effects of Chloramine-T,mainly with respect to the biochemical parameters in various tissues of grayling.

Keywords: Chloramine-T, gills, muscle, liver, heart, alanine aminotransferase, aspartate aminotransferase.


V.A. Chaplygin
Caspian Research Institute of Fisheries and oceanography
Astrakhan, Russia

A.S. Tarasova
Astrakhan State Technacal University 
Astrakhan, Russia

T.S. Ershova
Astrakhan State Technacal University 
Astrakhan, Russia

V.F. Zaitsev
Astrakhan State Technacal University 
Astrakhan, Russia

THE STUDY OF PECULIARITIES OF ACCUMULATION OF ZINC AND MERCURY BY AQUATIC ORGANISMS OF THE CASPIAN SEA

Abstract.Studied zinc and mercury migration features in components of the food chain of the Caspian Sea. It has been established that zinc does not accumulate in the food chain of the Caspian Sea ecosystem, whereas mercury migration hydrobionts occurs with increasing trophic level.

Keywords: aquatic organisms, mercury, zinc, bioaccumulation, bentofag.


SHIP POWER PLANTS, EQUIPMENT AND SYSTEMS, SHIP NAVIGATION FACILITIES, SHIP ELECTRICAL FACILITIES


V.V. Kiryuha
Far Eastern State Technical Fisheries University
Russia, Vladivostok

Yu.M. Gorbenko
Far Eastern Federal University
Russia, Vladivostok

A.Yu. Sashenko
Far Eastern Federal University
Russia, Vladivostok

V.S. Yablokova
Far Eastern Federal University
Russia, Vladivostok

THE ADJUSTING METHOD OF THE SOLUTION OF THE PROBLEM OF ELECTRICAL CIRCUITS DIAGNOSTICS

Abstract. An algorithm for diagnosing electric circuits at inaccurate measurements and noise parameters according to the least squares technique. The algorithm provides the correction of the solution based on theexact implementation of the component equations. Recommendations are given on the formation of a systemof equations and formulas are obtained for determining the value of the corrective coefficient.

Keywords: electric circuit, diagnosing, least squares technique, corrective coefficient, component equations.


TECHNOLOGY AND QUALITY CONTROL OF FOOD PRODUCTS


E.V. Glebova
Far Eastern State Technical Fisheries University
Russia, Vladivostok 

V.S. Panachina
Far Eastern State Technical Fisheries University
Russia, Vladivostok

DEVELOPMENT OF THE METHODICAL APPROACH TO FORMING THE INDICATORS OF THE SURVEY OF THE SUPERVISORY ACTIVITY, FOR THE COMPLIANCE OF THE MANDATORY REQUIREMENTSOF THE FOODSTUFFS INSTALLED

Abstract. Elements of increasing the effectiveness of supervision activities over observing the mandatory requirements established for food products are considered. With the help of the IDEF0 notation, a contextualdiagram of the implementation of the administrative procedure for checking compliance with themandatory requirements for kvass bread was constructed. As a result of the decomposition of the contextualdiagram with the help of IDEF0 notation, a functional model of conducting a scheduled on-site inspectionfor observing the obligatory requirements for kvass bread, which combined information flowsand control actions, was obtained. In the course of the work, the goal-goaling of the investigated objectwas decomposed, prior to dividing the lower-level objectives into simple tasks, the combined implementationof which would make it possible to achieve the global goal of the verification. Obtained simple actionsthat form the foundation of the goal tree, served as the basis for the formation of indicators of theeffectiveness of the procedure for conducting a routine on-site inspection of compliance with the mandatoryrequirements for kvass bread..

Keywords: state inspection, food products, mandatory requirements, technical regulations, process, process model, process goal, indicator of results.


E.V. Glebova
Far Eastern State Technical Fisheries University
Russia, Vladivostok 

V.V. Maksimova
Far Eastern State Technical Fisheries University
Russia, Vladivostok 

INTERRELATION THE CONCEPTS OF EFFICIENCY AND EFFECTIVENESS IN THE ASSESSMENT OF QUALITY MANAGEMENT SYSTEM

Abstract. The article defines the difference in the concepts of «efficiency» and «effectiveness» on the basis of their comparative analysis. Proposals for the separation of these concepts in relation to quality managementsystems are substantiated.

Keywords: efficiency, effectiveness, quality management system standards, assessment.


N.V. Dementeva
Far Eastern State Technical Fisheries University
Russia, Vladivostok

V.D. Bogdanov
Far Eastern State Technical Fisheries University
Russia, Vladivostok

O.V. Sakharova
Far Eastern State Technical Fisheries University
Russia, Vladivostok

EVALUATION OF THE NUTRITIONAL AND BIOLOGICAL VALUE OF MACKEREL AND TARP

Abstract. According to the results of the research showed that the mackerel and lingcod is a valuable food raw materials: study of the chemical composition of mackerel and Atka mackerel showed that mackerel incomparison with the rasp contains less protein and lipid and more water. A feature of mackerel meat is alow pH of meat, which is 5.5, probably due to the increased content in the protein amino acid – histidine.Fish are a rich source of biologically valuable protein, which contains all the essential amino acids.Quantitative amino acid content of fish is similar. Nevertheless, mackerel has a high content of histidineand isoleucine. These studies show that mackerel and lingcod biological values have strong positive characteristicssimilar to those of the reference protein casein. The relative biological value of mackerel is100.2%, in terpug - 89.5%. At all stages of the experiment, the ciliate was very active, and slowinggrowth, mutations and death of single cells was observed. On the basis of obtained data it is possible toassert with confidence that the mackerel and lingcod biologically safe raw material does not havemutagenic or toxic effects on a live cell of the ciliate Tetrahymena pyriformis.

Keywords: mackerel, terpug, chemical composition, amino acid composition, biological value.


N.N. Kovalev
Far Eastern State Technical Fisheries University
Russia, Vladivostok

E.I. Rybnikova
VIK Ltd
Russia, Vladivostok

CUCUMARIA JAPANESE AS A COMPONENT OF MEAT STUFFING PRODUCTS

Abstract. The choice of object of study due to the high biological activity of the component tissues of cucumaria Japanese. The relevance of the study is based on the property of triterpene glycosides to providebacteriostatic effect. Cucumaria introduced in meat products (sausages) cooked shredded tissue inan amount of 3% alcoholic extract (0.1 ml per 100 g of the total weight of the stuffing). The introductionof the tissue extract of cucumaria increased the shelf life of frankfurters up to 10 days at a temperatureof 0-6°C. In the sample of sausages with boiled meat of cucumaria there is an increase in all strengthcharacteristics during the storage process. The increasing values of dynamic modules are observed inthe sample prepared with the addition of alcohol extract of cucumaria, which indicates the most stablestructure of the studied samples of sausages throughout the shelf life. The influence of ethanol extract ofcucumaria on saprophytic putrefactive bacteria is estimated. It is shown that ethanol extract has an inhibitoryeffect on the growth of strains of putrefactive bacteria forming the film on the surface of sausagesduring storage.

Keywords: cucumaria japonica, sausages, alcohol extract, rheology, microbiological parameters.


N.L. Kornienko
Far Eastern State Technical Fisheries University
Russia, Vladivostok

L.B. Guseva
Far Eastern State Technical Fisheries University
Russia, Vladivostok

INTEGRATED USE OF RAW MATERIALS AS AN INNOVATIVE DIRECTION OF FISHERY INDUSTRY DEVELOPMENT

Abstract. Scientifically substantiated and carried out experimental studies characterizing the possibility of using fish broths from the secondary raw material of rudd and saffron cod in the technology of food products,including fish pates from crushed muscle tissue. This increases the degree integrated use of FarEastern fish and determines one the directions innovative development of fish processing enterprises. Thenon-linear nature the influence of the hydromodule and the duration of cooking on individual functionaltechnological(solids content, emulsifying ability, organoleptic properties) and colloidal (kinematic viscosity,surface tension) properties broths from Far Eastern fishes is established. Technological parametersof cooking broths are established, which ensure the formation optimal values of the functional andcolloidal properties broths necessary for the production high quality fish pâtés: for broths of red mud hydromodulesin the range from 0,5 to 1,5, with a cooking time of 60 minutes; for broths from saffron codthe hydromodule in a range from 1,0 up to 1,5, at duration cooking of 60 minutes.

Keywords: secondary raw materials, complex use, cooking mode, fish broths, functional and technological properties, organoleptic properties.


V.V. Krashenko
Far Eastern State Technical Fisheries University
Russia, Vladivostok

O.V. Osip
Far Eastern State Technical Fisheries University
Russia, Vladivostok

A STUDY OF THE INFLUENCE OF DIETARY FIBER FROM AQUATIC BIOLOGICAL RESOURCES ON THE QUALITY OF SEMI-FINISHED PRODUCTSIN THE MANUFACTURE OF DRYERS VANILLA

Abstract. The article presents the results of studies of the effect of dietary fiber from aquatic biological resources, namely sodium alginate and chitosan on the properties of baker's yeast. It has been establishedthat sodium alginate and water-soluble chitosan in the composition of the dough or test significantly influencethe activity and lifting power of the yeast.

Keywords: sodium alginate, water-soluble chitosan, drying, baking yeast, yeast activity, yeast lift.


M.V. Sytova
Russian Federal Research Institute Of  Fisheries and Oceanography (VNIRO)
Moscow, Russia

L.S. Abramova
Russian Federal Research Institute Of  Fisheries and Oceanography (VNIRO)
Moscow, Russia

L.H. Kotelnikova
Russian Federal Research Institute Of  Fisheries and Oceanography (VNIRO)
Moscow, Russia

RECOMMENDATIONS FOR THE MANAGEMENT OF TRACEABILITY AND LABELING RULES OF STURGEON FISH PRODUCTS

Abstract. The adoption of the "Strategy for improving the quality of food products in the Russian Federation until 2030" sets the task of managing the quality and safety of fish products on the basis of traceabilityand tightening the requirements for labeling to identify products. The solution to this problem will preventthe flow of illegal sturgeon products to the goods market. Recommendations for the rules of labeling sturgeonproducts, including caviar, were prepared, taking into account the requirements of Technical regulationsTR CU 022/2011 and technical regulations of the EAEU “on the safety of fish and fish products" TREAEU 040/2016 and other international documents. To identify fish products, bar coding or QR coding isthe most acceptable for fish raw materials both from the economic and technological point of view. Theanalysis made it possible to prepare recommendations for the management of traceability and labelingrules for sturgeon products in order to control the safety and quality of sturgeon food products at allstages of production, storage, transportation, processing, and sales, meeting the requirements of nationallegislation and international requirements.

Keywords: recommendations, traceability, labeling, bar-coding, production, aquaculture, sturgeon.